Molecular Cloning of Human beta-arrestin1 cDNA, Expression and Functional Study.

Complete coding sequences of β arrestin1 (1A and 1B) were cloned through application of bioinformatics analysis to the dbEST database. β arrestin1A was overexpressed in E.coli with partial expression products as inclusion body. Anti β arrestin1 antibodies were prepared by using purified inclusion body. Results also demonstrate that activation of inhibitory G protein mediated by δ and κ pioid receptors was strongly attenuated by overexpression of β arrestin1A in co transfected 293 cells.