Synthesis and spectroscopic characterization of a zinc oxide-polyphenol nanohybrid from natural resources for enhanced antioxidant activity with less cytotoxicity

Abstract Hydrothermal assisted Zinc Oxide (ZnO)-polyphenol nanohybrid (NHs) have been synthesized using natural products. The formation of nanohybrid was confirmed by UV VIS spectroscopy, fluorescence spectroscopy, and time resolved fluorescence spectroscopy. The crystalline morphology and size distribution was studied by TEM analysis. The synthesized zinc oxide (ZnO) nanohybrid exhibited antioxidant property by exerting excellent DPPH free radical scavenging activity. Whereas, no ROS generation was observed against DCFH. No inherent cytotoxicity of the synthesized nanohybrid was observed on 3T3L1 adipocyte cells. The study further demonstrated a cytoprotective activity of the nanohybrid on H2O2 induced oxidative stress on 3T3L1 cells indicating the potential therapeutic activity of the synthesized ZnO-Polyphenol nanohybrid.