Morphological and ultrastructural changes of the neuro-endocrine-immune network in the tumor transplantation animal model of abnormal Savda syndrome.

Objective:Abnormal Savda syndrome transplantation tumor model was established on the basis ofabnormal Savda syndrome model in order to study the relevance between the abnormal Savda syndrome tumor and the disorder ofthe neuroendocrine-immune network by analyzing morphological and ultrastructural changes ofthe hypothalamus-pituitary-adrenal axis(HPAA).Methods:120 ICR mice were divided randomly into four groups(control, tumor model, abnormal Savda syndrome model, and abnormal Savda syndrome tumor transplantation model).The structural changes in hypothalamus, pituitary and adrenal glands were analyzed by bright-field and electron microscopy.Results:First, in abnormal Savda syndrome, the rate oftumor transplantation was increased significantly than in the normal state(93.3% and 56.7%, respectively, P0.01);and the weight oftumor mass was also higher in abnormal Savda syndrome compared with the normal controls(P0.01).Secondly, cells ofthe hypothalamus were characterized by glial cell hyperplasia, neural cell edema, and vacuole degeneration in all three experimental groups among which the abnormal Savda syndrome tumor model showed the most severe symptoms.Pituitary cell hyperplasia and vascular dilation were similar in all three experimental groups.Thirdly, all three experimental groups had HPAA ultrastructural abnormalities including nuclear malformation, perinuclear cisterna enlargement, heterochromatin condensation, and cellular organelle degradation, but the abnormal Savda syndrome tumor model had the most severe phenotypes followed by the abnormal Savda syndrome group.Conclusions:Abnormal Savda syndrome body fluid facilitated tumor development, a phenomenon that might be related to the neuroendocrine-immune disorder.HPAA damages in abnormal Savda syndrome tumor model were more severe than the other experimental groups, suggesting that abnormal Savda syndrome body fluid and the tumor might have a synergistic effect on HPAA damages.The disturbance ofthe neuroendocrine-immune axis in abnormal Savda syndrome tumors was more severe as shown by the morphological data.