Versatile, Adaptable and Programmable Microfluidic Platforms for DNA Diagnostics and Drug Discovery Assays

A PCR DNA diagnostics system based on a hybrid microfluidic platform is described which offers the advantage of disposability for high DNA concentration PCR. PCR results for a 100 μ1 solution of M13 Bacteriophage DNA as well as a β-Actin human DNA TaqMan® assay show positive amplification after automated sample preparation in the block for both cases. Results have also shown successful follow-up decontamination of the reusable platform after each sample preparation and amplification run. The design and processes utilized in this hybrid system has been leveraged to design and develop a fully disposable cartridge platform for detection of Chlamydia and Gonorrhea from urine samples.