Synthesis of H-Lys-Lys-Gly-OH Tripeptide and Study of Its Acute Toxicity

Tripeptide H-Lys-Lys-Gly-OH was synthesized. Its toxic properties and biological activity were studied. The tripeptide was synthesized by extending the peptide chain from the C-terminus by synthesizing N2, N6-dicarbobenzoxylysine [N2,N6-(Cbz)2Lys] and N6-carbobenzoxylysine (N6-CbzLys) methyl ester hydrochloride and condensing them by the mixed anhydride method to produce the methyl ester of N2,N6-(Cbz)2Lys-N6-CbzLys. The next step in the tripeptide synthesis consisted of obtaining glycine benzyl ester (Gly-OBzl) hydrochloride and condensing it with N2,N6-(Cbz)2Lys-N6-CbzLys hydrazide to produce the protected target tripeptide Z-Lys(Z)-Lys-Gly-OBzl. The last step consisted of removing the protection using hydrogenolysis over Ni-Re catalyst. Test results showed that the synthesized tripeptide had low toxicity.