Large-Scale Mammalian Cell Cultures by Application of Macroporous Cellulose Carriers
1992
The large-scale culture of genetically engineered CHO cells has been demonstrated using collagen coated macroporous cellulose carriers in a 7.0 L stirred perfusion reactor. The results showed that the high cell density exceeding 107 cells/mL, or 2 × 109 cells/g-carrier was achieved over 20 days at a perfusion rate of 0.45-0.75 day-1.
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