Cloning, characterization and expression analysis of GbWRKY11, a novel transcription factor gene in Ginkgo biloba.

WRKY transcription factors are involved in diverse life processes in plants. Thus far, these proteins have not been reported in Ginkgo biloba. In this study, we cloned GbWRKY11, a novel WRKY transcription factor gene, from G. biloba. The cDNA of GbWRKY11 is 1,707 bp in length and encodes a putative protein of 402 amino acids. The GbWRKY11 protein has an estimated molecular weight of 43.64 kDa and belongs to the WRKY IId group. The genomic DNA of GbWRKY11 contains two introns and three exons. GbWRKY11 gene is ubiquitously expressed in all tested tissues but preferentially expressed in female flowers. The GbWRKY11 transcript is upregulated in response to salicylic acid, ethephon, and abscisic acid but repressed by methyl jasmonate, salinity, cold and heat. Thus, GbWRKY11, a newly identified G. biloba WRKY transcription factor, is apparently involved in multiple signaling pathways in response to abiotic stresses. This study provides a basis for further research on the function of GbWRKY11. © 2016 Friends Science Publishers