Animal brucellosis competitive ELISA (enzyme linked immunosorbent assay) antibody detection kit

The invention relates to a fast, efficient and accurate animal brucellosis diagnosis method. On one hand, the method adopts a 3E4 strain monoclonal antibody specifically combined with brucella; in brucellosis antibody detection, the monoclonal antibody 3E4 strain can generate specific competition with an antibody generated by the brucella, but does not compete with other pathogenic bacteria, and particularly does not compete with enterocolitis yersinia O9 and escherichia coli O157 antiserum in a strong cross reaction with brucella; the technical defect that the existing commercialized brucellosis competitive ELISA (enzyme linked immunosorbent assay) kits can not distinguish the interferences of enterocolitis yersinia O9 from escherichia coli O157 is effectively overcome, and the detection specificity is improved; and on the other hand, the kit provided by the invention adopts brucella suis (S2 strain) lipopolysaccharide (LPS) with good reactionogenicity with pigs, cattle and sheep as an envelope antigen, and increases the output of LPS, reduces the production cost and improves the detection sensitivity.