Assignment of the equine colony stimulating factor 1 receptor gene (CSF1R) to equine chromosome 14q15→q16 (ECA14q15→q16) by in situ hybridization and radiation hybrid panel mapping

The receptor of the macrophage colony-stimulating factor 1 (CSF1R) belongs to a subfamily of receptor tyrosine kinases (Roberts et al., 1988; Yarden and Ullrich, 1988). The CSF1R gene is in close vicinity to the PDGFR1 gene (platelet-derived growth factor receptor 1) which is located only a few hundred basepairs proximal in a head-to-tail orientation. Hampe et al. (1989) demonstrated that the human CSF1R gene consists of 21 small exons interrupted by introns ranging in size from 6.3 to less than 0.1 kb. Mutations in the CSF1R gene have been shown to be causative for a number of hereditary diseases and neoplastic transformations (Ridge et al., 1990; Boultwood et al., 1991). We have isolated and characterized the complete equine CSF1R gene. In agreement with the human ortholog, the equine CSF1R gene harbours 21 exons coding for a protein of 968 amino acids and spans a region of approximately 30 kb. Materials and methods