Synthesis of N-[N-(4-deoxy-4-amino-10-methylpteroyl)-4-fluoroglutamyl]-.gamma.-glutamate, an unusual substrate for folylpoly-.gamma.-glutamate synthetase and .gamma.-glutamyl hydrolase

N-[N-(4-deoxy-4-amino-10-methylpteroyl)-4-fluoroglutamyl]-γ-glutamate has been synthesized and its ability to serve as a substrate for folylpolyglutamate synthetase and γ-glutamyl hydrolase has been investigated. It was anticipated that this compound would be a substrate for both of these enzymes