High mobility group in A1a (HMGA1a) small interfering RNA (siRNA) blockage decreases metastatic potential in cultured osteosarcoma cell lines

High mobility group A1 (HMGA1) is a non-histone chromosomal nuclear protein that plays important roles on gene transcription, recombination, and chromatin structure stabilization. The aim of this study was to investigate the effect of small interfering RNA (siRNA) on HMGA1 expression and metastatic potential in osteosarcoma cell in vivo. We obtained osteosarcoma cell sublines after single cell cloning technique from human osteosarcoma cell line MG-63, and chose the cell subline with high HMGA1 expression. One sequence-specific siRNA targeted to HMGA1 was designed and cloned to generate the pU6mRFP-HMGA1 shRNA vector, then transfected into the osteosarcoma cell subline. The transfection efficiency of the experiment group and control group were 55.68±6.74 and 49.87±4.33%. The specific HMGA1 shRNA effectively down regulated the mRNA and the protein expression of osteosarcoma cell subline. The cells numbers throwing membrane at the experiment group was higher than that of the control group. shRNA of HMGA1 could successfully inhibit the expression of target gene in mRNA and protein levels and inhibit the in vitro metastatic potential of osteosarcoma cells.