Metabolic pathways of OGCP and the influence of parkin protein on the metabolism of OGCP

Objective To study the metabolic pathways of 2-oxoglutarate carrier protein(OGCP)and the influence of parkin protein on the metabolism of OGCP.Methods The OGCP metabolic pathways were identified through inhibiting proteasome activities with specific proteasome inhibitors and protease inhibitors.The isotope pulse-chase experiments were performed to measure the turnover rate of OGCP and to study the influence of parkin protein on the metabolism of OGCP.Results Proteasome inhibitors and protease inhibitors inhibited OGCP degradation.The OGCP metabolism had a half-life of about 8-10 h.Overexpression of parkin protein accelerated the OGCP degradation.Conclusion OGCP degrades through proteasome and lysosome degradation pathways.The degradation of parkin protein can promote the degradation of OGCP.