Diphtheria anatoxin industrial purification by gel filtration

Diphtheria toxoid is prepared from the toxin produced by fermentative process, of the Park-Williams N§8 strain of Corynebacterium diphteriae grown on F-20 medium. The toxin separeted from the organisms is detoxified and purified. Diphtheria toxoid is the immunizing antigen protecting against the adverse effects of infection by diphtheria organisms and rarely is used alone. It is used in associated vaccines with tetanus toxoid and pertussis vacine. This paper describes the separation of diphtheria anatoxin proteins on Sephadex G-50, Sephacryl S-100 HR and Superdex 75 prep grade