Resumption of Meiosis-I After Transfer of Mouse Primordial Oocytes from Frozen-Thawed Ovarian Tissue to Enucleated Preovulatory Oocytes: A Preliminary Report

Purpose: Ovarian tissue banking may be the best strategy to preserve female fertility. But optimal method to obtain viable mature oocytes remains challenging. In order to bypass the long in vitro oocyte growth period, we developed this study to test whether reconstruction of thawed primordial oocytes with enucleated preovulatory germinal vesicle (GV) oocytes could induce dictyate nuclei to undergo chromosomal condensation and meiotic maturation.