PREPARATION AND APPLICATION OF AFLATOXIN B_1(AFB_1) ANTI-IDIOTYPE ANTIBODY II. APPLICATION OF ANTI-AFB_1IDIOTYPE ANTIBODY
2004
Non-toxin ELISA was developed with fragment antigen-binding(Fab2) of anti-idiotype antibody (Ab2)substituting aflatoxin B1(AFB1). The specificity, AFB1 recovery in spiked samples and shelf-life of the non-toxin ELISA kit were investigated, and the kit was used in detecting AFB1 from agricultural commodities and feed. The results revealed that the non-toxin ELISA kit had the same specificity and AFB1 recovery ability with the regular ELISA kit, and both ELISAs could be used in detecting AFB1 from agricultural commodities and feed. The non-toxin ELISA kits were stored in 40C for three months and -200C for 5 months after appropriate treatment, the OD490nm values of kits in indirect ELISA were 85% and 87% of original OD490nm values, respectively.
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