Indomethacin suppress the proliferation and invasion of human choroidal melanoma cells

Objective To observe the influence of the indomethacin on the proliferative and invasive activity of OCM-1 human choroidal melanoma cells. Methods OCM-1 cells were cultured with different concentrations of indomethacin (25, 50, 100, 200, 400 μmol/L ), and their proliferation were assessed by methyl thiazolyl tetrazolium(MTT), invasive behaviors were examined by cell invasion assays, expression of survivin and VEGF were evaluated by reverse transcriptase polymerase chain reaction (RT-PCR),immunofluorescence staining, ELISA and western blot analysis. Result All concentrations of indomethacin in this study can inhibit the proliferation and invasion of OCM-1 cells in a time and dosage-dependant manner (MTT/24 h: F= 19. 642, P＜0.01;MTT/48 h: F= 136. 597, P＜0. 01;MTT/72 h: F= 582. 543, P＜ 0. 01;invasion assays: F= 54. 225, P＜ 0. 01). Immunofluorescence staining indicated that survivin and VEGF mainly expressed in the cytoplasm of OCM-1 cells. Survivin mRNA in OCM-1 cells was inhibited by 100,200, 400 μmol/L indomethacin (F= 16. 679, P＜0.01). The concentrations of survivin were (787.3±47.37), (257.0± 26.21), (123. 3± 8.02) pg/ml in control group and 100, 400 μmol/L indomethacin groups, respectively. Survivin expression was also significantly down-regulated in indomethacin-treated cells by Western blot analysis. Indomethacin had no effects on VEGF expression in OCM-1 cells. Conclusions Indomethacin can inhibit proliferation and invasion of OCM-1 cells in vitro, down-regulated expression of survivin may be the mechanism. Key words: Choroid Neoplasms; Indomethacin; Indomethacin/administration & dosage; Cell proliferation/ drug effects