Application of RAPD-PCR Markers for Identification and Genetic Analysis of American Elm (Ulmus americana L.) Selections

Abstract We used randomly amplified polymorphic DNA (RAPD) markers from DNA polymerase chain reactions to differentiate selected American elms. DNA profiles made from leaf DNAs of parental trees were identical to profiles produced by DNA from leaves, roots, vascular tissues, callus or suspension-cultured cells from the same trees or their ramets. In addition, several variations in the technique did not appear to alter the genotype-specific DNA profiles from a given elm selection. RAPD polymorphisms were inherited as dominant Mendelian factors in the progeny of a cross between two American elm selections. We conclude that the construction of RAPD DNA profiles is a reliable and easy method for selection identification, genetic analysis, and the assembly of linkage maps in American elms.