Releasing of intracellular glucoamylase from Aspergillus niger.

Abstract A method is presented for the release of glucoamylase (alpha-D-1,4-glucan glucohydrolase, E. C. 3,2,1, 3) from mycelia of Aspergillus niger. The process involves (a) suspension of mycelia at the stationary phase in water, and (b) homogenization and agitation of the suspension. The released intracellular enzyme is further purified by DEAE-cellulose.