The associated analysis of ERCC1 gene polymorphism in regulatory region and mRNA Q-PCR expression.

Objective:To investigate the distribution of SNP of rs3212986 and rs3212948 in ERCC1(excision repair cross-complementing group1)regulatory region in non-small cell lung cancer(NSCLC)compared with that of normal controls,as well as the association analysis between mRNA expression of ERCC1 gene and genotypes.Methods:TaqMan-PCR was used to detect the genotypes and allele frequencies of single-nucleotide polymorphisms(SNPs)of ERCC1 gene(rs743571,rs3212986,rs3212948)in 50 cases of paraffin-embedded NSCLC tissues and 10 cases of non cancer blood samples.SYBR Green real-time PCR was used to determine mRNA expression of ERCC1 which was associated with their ERCC1 SNP genotypes finally.Results:The result of Real-time PCR showed low expression of ERCC1 mRNA in 70% of NSCLC tissues compared to germline blood sample.The genotype and allele frequency of ERCC1 rs3212948 was significantly different(P0.05)in lung cancer group compared with that of control group,but this trend was not observed for rs3212986.The associated analysis of ERCC1 genotype showed there was no significant between regulatory region genotypes and mRNA expression.Conclusion:rs3212948 polymorphism in ERCC1 may contribute to the etiology of NSCLC;however,the significant association of the mRNA expression of ERCC1 with the genotypes of the two SNPs above has not been found.