Goat in vitro follicular response to insulin concentration is affected by base medium and follicular stage

Abstract Different culture media are used for the culture of preantral (PFs) and antral (AFs) follicles, among them the αMEM and TCM199. Also, culture media supplementation with insulin may improve the in vitro development of goat PFs and AFs. However, there is no information on the indicated concentration of insulin and which basis culture medium is the most suitable for caprine PFs and AFs. This study aimed to assess if the culture of PFs and AFs can be affected by the basis medium, and if its supplementation with two different insulin concentrations will improve follicular growth and oocyte development. Isolated PFs and AFs were in vitro cultured for 18 days in αMEM, or TCM199 supplemented either with 10 ng/mL or 10 μg/mL insulin. A higher degeneration rate (P