P-Glycoprotein Activity in Healthy Human Volunteers

were administered single oral dose of carbamazepine (200 mg) and digoxin (0.5 mg) before and after 10 17 days of honey (10 ml twice daily) intake. Blood samples (5ml) were collected at 0, 0.25, 0.5, 0.75, 1.0, 18 1.5, 2, 4, 8, 12, 24, 48 and 72 h after drug administration. Concentration of carbamazepine and digoxin in 19 plasma was measured by HPLC and RIA method respectively. Ten days of honey administration did not 20 significantly alter the C max, Tmax and AUC (0-t) of carbamazepine (probe drug for CYP3A4) and digoxin 21 (probe drug for P-gp). Our results suggest that honey may not significantly modulate the CYP3A4 enzyme 22 and P-glycoprotein activity. The coadministration of honey with drugs may not result in significant drug 23 interactions. 24