Streptomyces lushanensis sp. nov., a novel actinomycete with anti-cyanobacterial activity

Strain JXJ 0135T, an anti-cyanobacterial actinomycete, was isolated from a soil sample collected from Lushan Mountain, south China, and identified by using polyphasic approach. Phylogenetic analysis of the near-complete 16S rRNA gene sequence indicated that strain JXJ 0135T belongs to the genus Streptomyces and exhibited distinct subclade and also highest similarity (98.6%) to Streptomyces scopuliridis RB72T. The strain developed well-branched substrate and aerial mycelia, and produced spiral spore chains. Spores were elliptical and the spore surface was smooth. The strain contained LL-diaminopimelic acid with whole-cell sugars of mannose, rhamnose, glucose and galactose. Phospholipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol mannosides, phosphotidylinositol dimannoside, an unidentified aminophospholipid and an unknown phospholipid. The menaquinones were MK-9(H6) and MK-9(H8). The major components of the fatty acids were anteiso-C15:0, iso-C16:0, anteiso-C17:0, iso-C15:0, C16:0, iso-C17:0 and iso-C14:0. The G+C content was 69.3 mol%. The DNA–DNA hybridization value between JXJ 0135T and S. scopuliridis RB72T was 41.2±1.4%. On the basis of the polyphasic data, strain JXJ 0135T represents a novel species of the genus Streptomyces, for which the name Streptomyces lushanensis sp. nov. is proposed. The type strain is JXJ 0135T (=DSM 42121T=JCM 19628T=KCTC 29261T=KACC 17834T=NRRL B-24994T).