Isolation and Determination of Candida albicans on The Mouth and Siwak Salafi Community

Siwak is a Salvadoraceae family that is reported by some researchers to have an antimicrobial effect. The purpose of this study was to determine Candida albicans in the mouth and siwak. This research is a type of "true experimental" research with the population of the Salafi community of the Lima Kaum Batusangkar mosque, the sample was taken by purposive sampling as many as three people who showed different ways of using it. The stages of the study were divided into 2 steps, the first step was the isolation of Candida albicans in all three types of samples, the second step was by molecular identification, DNA extraction was carried out by following standard procedures from Sambrook using the phenol-chloroform technique. The Polymerase Chain Reaction (PCR) process was carried out using a Thermal Cycler Applied Biosystem Type 2700 with a volume of 25 µl containing 100ng/ml of total DNA, 2 µl 2.5 mM dNTP, 0.62 µl (10 mmol) primary mix forward, and 0.625 µl 10x buffer. The results of the amplification of CO1 gene fragments in electrophoresis using an agarose gel. Readings of the CO1 gene sequences were carried out in Macrogen Co., Korea, using forward primers MI3F (-21) 5'TGTAAAACGACGGCCAGT3 'and reverse primer MI3R (-27) 5'CAGGAAACAGCTATGAC3'. The results showed that the isolation of the mouth and wood of washed and unwashed siwak showed positive Candida albicans while in samples that varied the use of siwak and toothpaste there was no Candida albicans found in the mouth and the wood of the siwak.