Loss of the Protein NUPR1 (p8) Leads to Delayed LHB Expression, Delayed Ovarian Maturation, and Testicular Development of a Sertoli-Cell-Only Syndrome-Like Phenotype in Mice

Abstract The high mobility group factor NUPR1, also known as p8 and com1, plays a role in temporal expression of the beta subunit of luteinizing hormone, LHB, during gonadotroph development. At Embryonic Day (e) 16.5, LHB is detectable in wild-type (Nupr1+/+) but not Nupr1 knockout (Nupr1–/–) mice. LHB is initiated by e17.5 in Nupr1–/– mice, and expression is fully recovered by Postnatal Day (p) 2. Factors indicative of pituitary maturation, GATA2, CGA, and TSH, are not differentially expressed in Nupr1–/– and Nupr1+/+ embryos at e17.5. Therefore, the delay in LHB expression does not appear to result from delayed pituitary development. In addition, the role of NUPR1 in gonadotropin expression appears specific for LHB, as no difference in FSHB is observed in Nupr1–/– and Nupr1+/+ embryos. The gonads are also impacted by the absence of NUPR1. Ovaries of female Nupr1–/– mice lack corpora lutea (CL) at 8 wk, an age at which CL are present in all Nupr1+/+ littermates. Sexual maturity is recovered by 11 wk in N...