Expression and Identification of IBDV VP3 Structural Protein in E.Coli

The VP3 gene about 893bp was obtained from the total RNA of the chicken IBDV by RT-PCR, then the VP3 gene was subcloned into the downstream of T7 promoter of the vector pET-28a to construct expression vector of pETVP3. The recombinant plasmid was transformed into in E.coli BL21(DE3) and induced with IPTG. SDS-PAGE and Western blotting analyses showed that the chicken IBDV VP3 protein of 35.5 and 33 kDa were efficiently expressed and mainly existed as inclusion bodies,with specific reactivity to anti-IBDV antibodies.