MHC-DRB exon-2 (BuLA-DRB3) polymorphism in Banni breed of Indian buffalo.

Polymorphism in the DRB3- exon 2 of the BoLA (BuLA) gene of the Banni buffalo breed was studied by the technique of PCR-RFLP. The PCR product (304 bp) was digested with three enzymes RsaI, BstYI and HaeIII. The digestion products were separated on native PAGE gels which were silver stained. The results obtained showed presence of 6 different BuLA-DRB3.2 alleles viz. BuLA –DRB3.2* 19, *20, *36, *41, *abd and *gbb.