Immunotypin g of Lymphom a

try. Light-chain immunoglobulin ratios (K/X or X/K) obtained by both methods proved to be reliable independent predictors of polyclonality or monoclonality. Correlation studies using the Spearman rank coeffi­ cient revealed good concordance among values of K, a, CD3, and CD5 obtained by the two techniques, suggesting that subjective quantitation by cytospins yields similar results to objective quantitation by flow cytometry. Cytospin analysis and flow cytometry appear equally capa­ ble of immunotyping aspirated lymphoid samples reliably. The advan­ tages of each method are discussed. (Key words: Correlation; Cyto­ spins; Fine-needle aspiration; Flow cytometry; Immunotyping; Lymphoma) Am J Clin Pathol 1994;101:569-576.