SSR Analysis of the Variants from Rice Maintainer Lines Transferred Exogenous DNA

The new CMS line Yewei A and its maintainer line Yewei B were developed from the variant of V20B, a very popular rice maintainer line, into which the genomic DNA of O.minuta was transformed. Three variants were also selected from another maintainer line, IR58025B, after the introduction of the genomic DNA of sorghum. From the D_2 generation of one of the three variant plants on, no morphological segregation has been found among its different filial plants.This variant is denoted as ″Xiangliang 5″. The results of SSR analysis showed that there existed much polymorphism of repetitive DNA sequence between the variants and the receptors, with special DNA segments from distant relatives (donors) integrated into the genome of rice. The paper has first proven at the molecular level that some variants from transformation of exogenous DNA can be stablized in the second generation. It is also concluded that introduction of the genomic DNA from distant relatives is an effective way to create new germplasms in rice.