[Expression, purification, antibody preparation and identification of tumor protein translationally-controlled 1 (TPT1)].

AIM:To express and purify the fusion protein of TPT1(tumor protein translationally-controlled 1) in prokaryocytes and to prepare rabbit anti-TPT1 antibody.METHODS:The expression vector pRSETA2-TPT1 was reconstructed and transformed into BL21(DE3).TPT1 fusion protein was induced by IPTG and the TPT1 fusion protein purified by Ni-NTA His Bind resin was used to immunize the rabbit.The titer of the polyclonal antibody was detected by ELISA and its specificity was analyzed by Western blot and IF(immunofluorescence).RESULTS:The TPT1 fusion protein was highly expressed in E.coli and specific polyclonal antibody was obtained after the immunization.CONCLUSION:The recombinant prokaryotic expression vector pRSETA2-TPT1 is successfully constructed and high expression of TPT1 is induced in E.coli,which may lay the basis for further study of the development and treatment of tumors and other diseases.