Pyrolysis solution, extracting solution, pyrolysis and extraction method, kit and application and PCR (Polymerase Chain Reaction) system

The invention discloses a pyrolysis solution, a DNA extracting solution, a pyrolysis and extraction method, a NDA extraction kit and application and a PCR (Polymerase Chain Reaction) system. The pyrolysis solution is used for splitting fish tissue to release DNA, and comprises ethylenediamine tetraacetic acid and/or ethylenediamine tetracetic acid disodium salt, sodium chloride, lauryl sodium sulfate, strong base and water, wherein the mole concentration of the ethylenediamine tetraacetic acid and/or the ethylenediamine tetracetic acid disodium salt is 2-10mM relative to the pyrolysis solution; the mole concentration of the sodium chloride is 1-10mM relative to the pyrolysis solution; the mass percentage of the lauryl sodium sulfate is 0.1-10% relative to the pyrolysis solution; the mole concentration of hydroxyl ions of the strong base is 100-300mM. Compared with the prior art, the pyrolysis solution disclosed by the invention is capable of providing a DNA template for fish tissue PCR amplification within relatively short time, reduction or loss of sample DNA can be avoided in the whole process, and moreover, operation is simple and convenient and easy to master.