Knockout of SlMAPK3 Reduced Disease Resistance to Botrytis cinerea in Tomato Plants

Mitogen-activated protein kinases (MAPKs) play an important role in defense responses to biotic and abiotic stresses. In order to investigate the role of SlMAPK3 in tomato plant resistance to Botrytis cinerea, two lines of slmapk3 mutants and wild-type (WT) tomato plants were used. The results showed that slmapk3 mutants were more susceptible to B. cinerea and that knockout of SlMAPK3 reduced the activities of defense enzymes and enhanced the accumulation of reactive oxygen species (ROS). Furthermore, we detected the expressions of salicylic acid (SA) and jasmonic acid (JA) signaling-related genes and found that knockout of SlMAPK3 enhanced the expressions of SlPR1, SlPAD4 and SlEDS1, whereas reduced the expressions of SlLoxC, SlPI I and SlPI II and enhanced the expressions of SlJAZ1 and SlMYC2. We postulate that SlMAPK3 plays a positive role in tomato plant resistance to B. cinerea through regulating ROS accumulation and SA and JA defense signaling pathways.