Application of SRAP Markers to the Identification of Eremochloa ophiuroides(Munro) Hack Hybrids

The hybrids status from two hybridization combinations of Eremochloa ophiuroides(Munro) Hack with four parents(E022,E142,E102,E092(1)) were tested using SRAP primer combinations with clear and stabilized paternal characteristic bands selected from 400 primer combinations.The results show:(1) 51 primer combinations with paternal characteristic bands were selected in A(E102×E092(1)) and 87 primer combinations were selected in B(E142×E022).(2) Six primer combinations(303,297,283,180,332 and 358) were used to identify the hybrids status in A(E102×E092(1)) and of which,89 were true hybrids.Four primer combinations(18,199,221 and 288) were used to identify the hybrids status in B(E142×E022) and of which,83 were true hybrids.These true hybrids could be useful in the genetic linkage map construction and QTL mapping of important characters.