Anti-Vibrio Activity of Mangrove and Mangrove Associates on Shrimp Pathogen, Vibrio harveyi VSH5

The petroleum ether, chloroform, acetone, ethyl acetate, methanol and aqueous extracts of six different species of mangrove plants and four mangrove associates were tested in vitro for their anti-vibrio activity for which shrimp pathogen Vibrio harveyi VSH5 was used as test pathogen. Out of tested 96 curd extracts, acetone extract of Acanthus ilicifolius leaves (16.4 mm) showed more potent anti-vibrio activity than the other leaf extracts. Among the fruit and stem extracts, methanolic extracts of Rhizophora mucronata fruit, Avicennia marina stem and Avicennia ofcinalis stem rendered their maximum activities as 12.1 mm, 10.2 mm and 10.2 mm respectively. Methanol and acetone extracts of Thespesia populneoides leaves recorded its maximum activity in mangrove associates. The Minimum inhibitory concentration MIC of acetone extract of A. ilicifolius leaves against the test pathogen was calculated as 125 µg/ml. Further, the test extract gave five separated bands on Thin layer chromatography TLC which showed clear zone (anti-vibrio activity) either compounds in individual or in combination through bioautography.