AVALIAÇÃO DO REAL POTENCIAL INIBIDOR DE EXTRATOS ETANÓLICOS DE Ottonia martiana SOBRE Cylindrocladium spathulatum E Botrytis cinerea

Abstract The antifungal potential of anestesia,  Ottonia martiana  Miq. was reassessed by factorial design, in vitro testing of fungal mycelial growth compared to the pathogenic isolates  Cylindrocladium spathulatum,  causal agent of black spot onyerba mate, and  Botrytis cinerea  causal agent of gray-mold on eucalypts. Occurrence of decomposition of bioactive of the autoclaving process was investigated using foliar detached test compared to the pathogens (1000 ppm). Ethanolic extracts - EBEtOH (filtered and autoclaved) inhibited the mycelial growth of  C. spathulatum  and  B. cinerea  (1000 ppm) and were more pronounced against  B. cinerea  (43.6 % and 68.9 %). EBEtOH filtered (0.22 µm) presented higher activity than EBEtOH autoclaved ( C. spathulatum : 52.8 % and 43.6 %,  B. cinerea : 68.9 % and 43.6 %), suggesting little decomposition ofbioactive after autoclaving. EBEtOH filtrate presented potential inhibition of 28 % in eucalypt leaves against  B. cinerea . Keywords :  Ottonia martiana;  black spot; gray-mold.