Predictive value of serum kisspeptin levels on 14 and 21 days after frozen-thawed embryo transfer

Abstract Research Question Whether the serum kisspeptin levels on 14 and 21 days after frozen-thawed embryo transfer (ET) could predict the early pregnant outcome of patients. Design Prospective study. 133 patients undergoing in vitro fertilization (IVF) and frozen-thawed ET enrolled. Patients are divided into non-pregnant (NP) group and pregnant group (including biochemical pregnancy (BP) group, single pregnancy (SP) group, Miscarriage group, and Twin group). Results Serum kisspeptin levels on day 21 are significantly higher than day 14 in SP group, Miscarriage group, and Twin group, but not in BP group. Similarly, serum HCG levels are higher on day 21 except for BP group. Compared to the Twin group (296.9 pg/ml), other four groups show significantly higher serum kisspeptin levels on day 14 (NP group 548.9, BP group 440.4, Miscarriage group 434.9, SP group 420.9, pg/ml, P P =0.016, P =0.034, P =0.036, respectively). Miscarriage group (762.2 pg/ml), SP group (730.8 pg/ml), and Twin group (826.3 pg/ml) have a significantly higher kisspeptin levels than BP group (397.3 pg/ml) on day 21 ( P P P Conclusions Serum kisspeptin levels on day 14 are negatively correlated with embryo implantation rate. Serum kisspeptin levels on day 21 have a poor predictive value of miscarriage.