Production and separation of exo- and endoinulinase from Aspergillus ficuum

Abstract The production of both exo- and endoinulinase by Aspergillus ficuum JNSP5-06 was investigated. Optimum fermentation conditions were found to be: inulin, 2%; yeast extract, 2%; (NH 4 )H 2 PO 4 , 0.5%; NaCl, 0.5%; MgSO 4 ·7H 2 O, 0.05%; ZnSO 4 ·7H 2 O, 0.01%; initial pH 6.5. A new and convenient method was developed to separate the inulinases by native-polyacrylamide gel electrophoresis (PAGE). Eight protein bands were obtained. Three bands were identified as exoinulinase and two bands were endoinulinase using TLC and HPLC.