Properties of an anion-selective channel from rat colonic enterocyte plasma membranes reconstituted into planar phospholipid bilayers

Vesicles derived from epithelial cells of the colonic mucosa of the rat were fused to planar phospholipid bilayer membranes, revealing spontaneously switching anion-conducting channels of 50 pS conductance (at-30 mV with 200mm Cl− each side). The equilibrium selectivity series was I− (1.7)/Br− (1.3)/Cl− (1.0)/F− (0.4)/HCO 3 − (0.4)/Na ( 0.5 sec) was observed.