Light induced heme oxygenase 1 is suppressed by Bach1 in human skin keratinocytes
2010
Ultraviolet A (UVA) radiation is an oxidizing agent that strongly induces the heme oxygenase 1(HO-1) expression in
cultured human skin fibroblasts, but weakly induces it in skin keratinocytes. Here, we report that low basal levels of HO-
1 and much higher basal levels of HO-2 protein were observed in keratinocytes compared with fibroblasts. Silencing of
Bach1 strongly increased HO-1 levels in HaCaT transformed keratinocytes and these HO-1 levels were not further
increased by either UVA irradiation or silencing of HO-2. This is consistent with the conclusion that high constitutive
levels of HO-2 expression in keratinocytes are responsible for the resistance of these cells to HO-1 induction by UVA
radiation and that Bach1 plays a predominant role in influencing the lack of HO-1 expression in keratinocytes. Bach1
inhibition reduced the 500 kJ/m 2 UVA-induced cell damage by LDH membrane integrity and MTS viability assays.
These results suggest that Bach1 inhibition protect against high dose of UVA irradiation induced damage in
keratinocytes.
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