Trehalose-Based Polyethers for Cryopreservation and Three-Dimensional Cell Scaffolds.

The ability to slow ice growth and recrystallization is compulsory in the cryopreservation of cells and tissues to avoid injuries associated with the physical and chemical responses of freezing and thawing. Cryoprotective agents (CPAs) have been added to the cell suspension to mitigate cryoinjury and increase cell survival but some of these compounds pose greater risks for the clinical application of cryopreserved cells due to their inherent toxicity. Trehalose is known for its unique physicochemical properties and its interaction with the phospholipids of the plasma membrane, can reduce cell osmotic stress and stabilized the cryopreserved cells. Nonetheless, there has been a shortage of relevant studies on the synthesis of trehalose-based CPAs. We here report and describe the synthesis and evaluation of a trehalose-based polymer and hydrogel and its use as a cryoprotectant and 3D cell scaffold for cell encapsulation and organoid production. In vitro cytotoxicity studies with the trehalose-based polymers ...