Investigating the chronic effects of oil sands process-affected water on growth and fitness of Daphnia magna Straus 1820

Abstract The increasing amount of stored oil sands process-affected water (OSPW), a primary by-product of oil sands mining, is an environmental concern. In the present study, we investigated the chronic effects of OSPW on growth, reproduction, and macronutrient content in Daphnia magna . To do so, we exposed D. magna to 1 and 10% OSPW (a mixture of three OSPW samples provided by major oil sands mining operators in northern Alberta) for ten days. We measured the number of the neonates produced daily in each group throughout the exposure. At the end of the exposure, we measured the mass and length of the exposed daphniids and neonates. We also measured the carbohydrate, lipid, and protein content of exposed daphniids. In the 10% OSPW group, we observed a significant reduction in all of the measured endpoints except for body length and carbohydrate and protein content of exposed daphniids. In the 1% OSPW group, on the other hand, we found a reduction only in lipid content of exposed daphniids as compared to the control group. The results of the present study demonstrated that chronic exposure to 10% OSPW affects growth and fitness of D. magna , probably due to a reduction in energy intake that causes daphniids to deplete their energy reserves.