Possibilities and Limitations of Photoactivatable Cytochalasin D for the Spatiotemporal Regulation of Actin Dynamics
2020
The study of the actin cytoskeleton and related cellular processes requires tools to specifically
interfere with actin dynamics in living cell cultures, ideally with spatiotemporal control and
compatible with real time imaging. A phototriggerable derivative of the actin disruptor
Cytochalasin D (CytoD) is described and tested here. It includes a nitroveratryloxycarbonyl
(Nvoc) photoremovable protecting group (PPG) at the hydroxyl group at C7 of CytoD.
The attachment of the PPG renders Nvoc-CytoD temporarily inactive, and enables light-dosed
delivery of the active drug CytoD to living cells. This article presents the full structural and
physicochemical characterization, the toxicity analysis. It is complemented with biological
tests to show the time scales (seconds) and spatial resolution (cellular level) achievable with a
UV source in a regular microscopy setup
- Correction
- Source
- Cite
- Save
- Machine Reading By IdeaReader
0
References
0
Citations
NaN
KQI