Effect of Duoluoqingtai Granula on expression of sinal transduction gene in human hepatocellular carcinoma cell line

Objective To investigate the effect of Duoluoqingtai Granula (DLQTG) on gene expression in human hepatocellular carcinoma cell line (SMMC-7721). Methods Effect of DLQTG on the growth of mice-transplanted hepatocellular carcinoma was studied in vivo and on the expression of signal transduction genes of SMMC-7721 cell line with serum pharmacology and microarray. Results DLQTG was observed to significantly affect the growth of mice-transplanted hepatocellular carcinoma with inhibition rate from 63% to 67.8%, and the expression of signal transduction genes in 68 of 99 genes detected with up-regulation of 42 genes and down-regulation of 36 genes. There was obvious difference in the expression of signal transduction genes in SMMC-7721 cell line between low and high dosage of DLQTG-treated group. Conclusion DLQTG can be used to inhibit the growth of hepatocellular carcinoma and to affect the expression of signal transduction genes in human hepatocellular carcinoma significantly.