Discovery of LRE1 as a specific and allosteric inhibitor of soluble adenylyl cyclase.

A high-throughput screen using a mass-spectrometry-based assay results in the identification of LRE1 as an inhibitor of HCO3−/Ca2+-regulated soluble adenylyl cyclase activity. LRE1 interacts with the HCO3− binding site (BBS) to block cAMP formation.