Coenzyme A and Coenzyme A‐Glutathione Mixed Disulfide Measurements by HPLC

This unit describes a method for measuring coenzyme A and coenzyme A-glutathione mixed disulfide in tissue homogenates obtained from tissues frozen in liquid nitrogen, homogenized in the presence of N-ethylmaleimide, and then acidified. to avoid the oxidation, reduction, and thiol exchange reactions that can occur during tissue processing and isolation of subcellular fractions. This method may provide a way to assess changes in the oxidative state of mitochondria in intact tissues.