ABSORPTION CORRECTED METHOD & ISOABSORPTIVE POINT METHOD FOR SIMULTANEOUS ESTIMATION OF METOPROLOL AND AMLODIPINE IN THEIR COMBINED DOSAGE FORM

Two simple, economical, precise and accurate methods are described for the simultaneous determination of Metoprolol Succinate (METO) and Amlodipine (AMLO) in combined tablet dosage form. The first method (Method A) is Absorption Corrected Method and second method (Method B) is Isoabsorptive Point Method. The absorbances at 277.017 nm and 235.62 nm in the Absorption Corrected Method and Isoabsorptive Point Method were selected to determine METO and AMLO, respectively in combined formulation. The methods were validated by following the analytical performance parameters suggested by the International Conference on Harmonization (ICH). All validation parameters were within the acceptable range. Under experimental conditions described, calibration curve, assay of tablets and recovery studies were performed. A critical evaluation of proposed methods were performed by statistical analysis of data where slope, intercept, correlation coefficient is shown in Table (1). As per the ICH guidelines, the method validation parameters checked. Beer’s law is obeyed in the concentration range of 50-250μg/ml and 5-25μg/ml for Metoprolol succinate and Amlodipine by both the methods.