Kebar grass (Biophytum petersianum K.) The effect inmaintaining mice (Mus musculus) sperm quality exposed to dioxin

Kebar grass contai ns active antioxida nts and potential vitamins to neutralize TCD D toxicity. Prove that Kebar grass extract in various dosage can main­ tain vi abil ity, motility, and sperm concentration of male mice exposed to 2,3,7,8-tetrach lorodibenzo-p-dioxin. This study was an experimental labora­ tory study with five groups consists of Negative Control (C-), Positive Control (K +) with TCD D exposure of 0.7µg/Kg BW I P single dose. The treatment group was given oral Kebar grass extra ct for 53 days treatment 1(Tl) with a dose of 0.045 mg / g BB, Treatment 2 (T2) with a dose of 0.08 mg/g/BW, and Treatmen t 3 (T3) with a dose of 0.135 mg/g/BW. The data of motility, via­ bility, and concentration of spermatozoa obtai ned were analyzed usi ng One Way Anova test and Duncan Multiple Range Test Ad minist ration of Kebar Grass Extract at a dosage of 0.135 mg/g/BW showed a significa nt difference between the control group and the treat ment group (p< 0.05). Exposure to TCD D in C+ decreased motility (13 ± 6.70%), viability (28 ± 19.35%), and concentration (0.87 ± 0.64 cells / mm3) of sperm significantly compared to C-. The administration of Kebar grass extract can mai ntain motility (74 ± 5.47%), viability (76 ± 2.72%), and concentration (2.50 ± 0.69 cells/ mm3) sperma­tozoa in the T3 group with a dose of 0.135mg/g/BW Kebar grass extract is effective for maintaining the quality of mice sperm from damage due to expo­ sure to 2,3,7,8-tetrachlorodibenzo-p- dioxin. Keywords: Kebar Grass, Motility, Viability