Development of a RT-PCR based method for identification of Anthurium andraeanum mosaic virus pathogen

The present research work has been undertaken to identify the pathogen(Dasheen mosaic virus,DMV) of Anthurium andraeanum mosaic virus using RT-PCR method,and to develop a reliable RT-PCR protocol for its detection,The method developed can detect the DMV pathogen in 500× diluted total RNA extracted from 0.1 g diseased tissues,which indicated very high sensitivity of this method for DMV detection.The establishment of DMV detection method will be helpful in commercial production of Anthurium andraeanum virus-free seedings.