Amino acid profile, Anti coagulant and Anti oxidant activities of coelomic fluid of the Earthworms Polypheretima elongata and Eudrilus eugeniae in relation to medical application

Production of pharmacologically important compounds from earthworms is a novel area in modern day medicine termed as vermiceuticals. In the present study effect of coelomic fluids of Polypheretima elongata and Eudrilus eugeniae were indeperndently examined for ome of their possible medical applications. Coelomic fluids from P.elongata and E.eugeniae were extracted by applying heat and cold shock treatments. The coelomic fluids of both the earthworm species showed anti oxidant and anti coagulant activities at different levels. Anti Coagulation assay was performed to the coelomic fluids of the earthworm species using standard clotting time delay method of Baughmann (1995). Both the species P.elongata and E.eugeniae exhibited the anticoagulant activity with the clotting time delay of maximum 60 and 40 Seconds respectively with the coelomic fluids volume of 200µl. Both the species exhibited antioxidant activity. The presence of Glutathione was confirmed in both the species at a concentration of 0.12 µg/ml and 0.06 µg/ml respectively in P.elongata and E.eugeniae. The above results clearly indicate that coelomic fluid of P.elongata has bioactive molecules in higher levels than E.eugeniae. The salient findings of the study include coelomic fluid of earthworms namely P.elongata and E.eugeniae has protein factors and some of the essential amino acids which have anticoagulant and antioxidant activity. Further research on the principal active components of coelomic fluids of two above mentioned earthworm species leads to development of drug against enhancement of wound healing property, to treat cancer, thrombolytic and hemophilic disorders in humans.