Study on material base of Carthamus tinctorius with antioxidant effect based on selective knock-out

Objective: To establish a method for studying efficacious materials of traditional Chinese medicines from an overall perspective. Method: Carthamus tinctorius was taken the example. Its major components were depleted by preparing liquid chromatography. Afterwards,the samples with major components depleted were evaluated for their antioxidant effect,so as to compare and analyze the major efficacious materials of C. tinctorius with antioxidant activity and the contributions. Result: Seven major components were depleted from C. tinctorius samples,and six of them were identified with MS data and control comparison. After all of the samples including depleted materials are compared and evaluated for their antioxidant effect,the findings showed that hydroxysafflor yellow A, anhydrosafflor yellow B and 6-hydroxykaempferol-3,6-di-O-glucoside-7-O-glucuronide were the major efficacious materials. Conclusion: This study explored a novel and effective method for studying efficacious materials of traditional Chinese medicines. Through this method,we could explain the direct and indirect contributions of different components to the efficacy of traditional Chinese medicines, and make the efficacious material expression of traditional Chinese medicines clearer.