Understanding the thermal denaturation of myoglobin with IMS-MS: evidence for multiple stable structures and trapped pre-equilibrium states

Thermal denaturation of holomyoglobin (hMb) in solution (10 mM ammonium acetate at pH = 4.5, 6.8 and 9.0) was monitored by ion mobility spectrometry (IMS) and mass spectrometry (MS) techniques to c...